Serial dilution design
A serial dilution repeatedly dilutes a sample by a fixed factor.
This calculator generates the expected concentration and practical transfer/diluent volumes for each tube.
Calculator
Serial Dilution Calculator
Result
Transfer volume each step: 100 µL
Diluent volume each step: 900 µL
Tube 1: 100 concentration units
Tube 2: 10 concentration units
Tube 3: 1 concentration units
Tube 4: 0.1 concentration units
Tube 5: 0.01 concentration units
Tube 6: 0.001 concentration units
Practical use
Use this calculator as a planning aid for molecular biology, biochemistry, protein science, cloning, PCR, and routine bench preparation workflows.
Results should be interpreted alongside your protocol, instrument settings, reagent quality, and laboratory safety requirements.
How to cite this tool
Citation
If this calculator supports your research, you may cite it as a web-based tool.
Abdulfattah, M. A. Serial Dilution Calculator. Mustafa A Abdulfattah Academic Website. Available at: https://www.mustafaabdulfattah.com/tools/serial-dilution-calculator. Accessed today.
BibTeX
@misc{abdulfattah_serial_dilution_calculator_2026,
author = {Abdulfattah, Mustafa A.},
title = {Serial Dilution Calculator},
year = {2026},
url = {https://www.mustafaabdulfattah.com/tools/serial-dilution-calculator},
note = {Accessed today}
}FAQ
Serial Dilution Calculator questions
What is a serial dilution?↑
It is a stepwise dilution where each tube is diluted by the same factor from the previous tube.
How is transfer volume calculated?↑
Transfer volume is final tube volume divided by the dilution factor.
What is diluent volume?↑
Diluent volume is final volume minus the transfer volume.
Can this be used for standards?↑
Yes. It is useful for standard curves, sample dilution planning, and assay setup.