Research

Engineering biological function with stability in mind

My research sits at the intersection of enzyme engineering, molecular biotechnology, and functional protein analysis, with a particular emphasis on thermostability enhancement, recombinant expression, and the characterization of industrially relevant enzymes.

Enzyme EngineeringThermostabilityMolecular Biotechnology

Publication

10+

Core Techniques

11+

Students Supervised

Years of Lab Experience

Research Focus

Core themes that define the scientific direction of my current and developing work.

Enzyme Thermostability

Investigating strategies to enhance enzyme stability under elevated temperatures in order to improve robustness, operational lifespan, and industrial relevance.

Protein Engineering

Designing and refining enzymes through domain fusion, structural modification, and function-oriented optimization approaches.

Industrial Biocatalysis

Exploring enzyme systems with practical value for scalable, efficient, and environmentally aligned biotechnological applications.

Current Work

Present experimental direction and technical emphasis.

My current work is centered on improving the thermostability of lipase enzymes through protein engineering strategies that combine rational thinking with experimental validation. The broader aim is to develop enzyme systems capable of stronger performance under demanding conditions relevant to biotechnology and applied research.

This includes cloning, recombinant expression, purification, and functional evaluation of target enzymes, alongside comparative analysis of how structural modifications may influence stability, activity, and overall usability.

Current emphasis

Stability-focused enzyme optimization and structure-guided comparison of promising design strategies.

Techniques and Expertise

Technical capabilities applied across molecular workflows, protein work, and analytical evaluation.

Protein ExpressionProtein PurificationHis-tag Affinity SystemsMolecular CloningEnzyme AssaysThermostability EvaluationSDS-PAGEDot BlotCell-based ELISACell CultureBuffer PreparationExperimental Optimization

Research Character

A concise view of how I approach scientific work beyond methods alone.

Research Direction

Focused on building more stable and functionally efficient enzyme systems through experimentally grounded engineering strategies.

Scientific Strength

Combines hands-on molecular biology with structured interpretation, technical refinement, and application-oriented thinking.

Working Style

Detail-oriented, methodical, and strongly motivated by clarity, reproducibility, and meaningful biological outcomes.

Publications

Research outputs connected to enzyme-focused molecular biology and biotechnology.

2024PublishedPLOS ONE

Characterization of a novel subfamily 1.4 lipase from Bacillus licheniformis IBRL-CHS2: Cloning and expression optimization

Ammar Khazaal Kadhim Almansoori, Nidyaletchmy Subba Reddy, Mustafa Abdulfattah, Sarah Solehah Ismail, Rashidah Abdul Rahim

This study presents a novel subfamily 1.4 lipase from *Bacillus licheniformis* IBRL-CHS2 and establishes an optimized recombinant expression system for producing the enzyme. The characterized lipase shows useful biochemical properties, including organic-solvent stability, supporting its potential value as a biocatalyst for industrial and biotechnological applications.

DOI View source