Mustafa Ali Abdulfattah
PhD Researcher / Molecular Biology / Enzyme Engineering
Malaysia (Primary) / Japan (Research Exchange)
Profile
PhD researcher working at the intersection of molecular biology, enzyme engineering, and applied biotechnology, with hands-on expertise in recombinant protein expression, purification, and functional enzyme characterization.
My research is centered on improving enzyme thermostability through structural modification and domain-fusion strategies, with the goal of developing robust biocatalysts for scientifically and industrially relevant applications.
My work combines experimental practice, scientific writing, and cross-environment research experience, including a JASSO-supported exchange in Japan.
1
Publication
4+
Years of Lab Experience
10+
Core Techniques
2
Research Environments
Techniques and Expertise
Technical competencies spanning molecular biology, protein expression, enzyme characterization, and research practice.
Molecular Biology
- Gene cloning and vector construction
- Recombinant DNA techniques
- PCR amplification and optimization
- Plasmid transformation
- DNA purification and quantification
Protein Expression and Purification
- Recombinant protein expression in E. coli
- IPTG induction optimization
- His-tag affinity purification
- Protein solubility optimization
- Buffer exchange and concentration
Enzyme Characterization
- Enzyme activity assays
- Thermostability analysis
- Kinetic parameter evaluation
- Substrate specificity assessment
- Reaction condition optimization
Analytical Techniques
- SDS-PAGE
- Protein quantification
- Dot blot
- Cell-based ELISA
- Spectrophotometric analysis
Cell Culture
- Mammalian cell culture
- Aseptic handling
- Cell viability assessment
Research Practice
- Experimental design
- Scientific data interpretation
- Scientific writing
- Literature review
Languages
Arabic / Native
English / B2
Current Research
Thermostability Enhancement of Lipase Enzymes
My current work focuses on engineering lipases from Bacillus pumilus and Burkholderia cepacia to improve thermostability through domain-fusion strategies and related structural approaches.
This research includes cloning, recombinant expression, purification, and enzymatic evaluation under varying conditions in order to better understand stability-function relationships and optimize performance.
Publications
Peer-reviewed and emerging outputs connected to my research direction.
Characterization of a novel subfamily 1.4 lipase from Bacillus licheniformis IBRL-CHS2: Cloning and expression optimization
Ammar Khazaal Kadhim Almansoori, Nidyaletchmy Subba Reddy, Mustafa Abdulfattah, Sarah Solehah Ismail, Rashidah Abdul Rahim
PLOS ONE / This study presents a novel subfamily 1.4 lipase from *Bacillus licheniformis* IBRL-CHS2 and establishes an optimized recombinant expression system for producing the enzyme. The characterized lipase shows useful biochemical properties, including organic-solvent stability, supporting its potential value as a biocatalyst for industrial and biotechnological applications.
Academic Direction
My broader research direction is centered on developing stable and functionally efficient enzymes for applied biotechnology, with particular interest in how structural engineering can improve performance under demanding conditions.