RNA concentration from A260
RNA concentration is often estimated from A260 absorbance using a common RNA conversion factor.
The dilution factor should reflect any dilution made before measuring the sample.
Calculator
RNA A260 Concentration Calculator
Result
RNA concentration: 40 µg/mL
Equivalent: 40 ng/µL
Practical use
Use this calculator as a planning aid for molecular biology, biochemistry, protein science, cloning, PCR, and routine bench preparation workflows.
Results should be interpreted alongside your protocol, instrument settings, reagent quality, and laboratory safety requirements.
How to cite this tool
Citation
If this calculator supports your research, you may cite it as a web-based tool.
Abdulfattah, M. A. RNA A260 Concentration Calculator. Mustafa A Abdulfattah Academic Website. Available at: https://www.mustafaabdulfattah.com/tools/rna-a260-concentration-calculator. Accessed today.
BibTeX
@misc{abdulfattah_rna_a260_calculator_2026,
author = {Abdulfattah, Mustafa A.},
title = {RNA A260 Concentration Calculator},
year = {2026},
url = {https://www.mustafaabdulfattah.com/tools/rna-a260-concentration-calculator},
note = {Accessed today}
}FAQ
RNA A260 Concentration Calculator questions
What factor is used for RNA?↑
A common estimate is A260 multiplied by 40 and the dilution factor for RNA concentration in micrograms per mL.
What does the dilution factor mean?↑
It accounts for dilution before measurement. An undiluted sample uses a dilution factor of 1.
Can degraded RNA affect the result?↑
A260 estimates nucleic acid amount but does not directly measure RNA integrity.
Should I check purity ratios?↑
Yes. A260/A280 and A260/A230 ratios help screen for possible contaminants.