DNA concentration from A260
A260 absorbance is commonly used to estimate nucleic acid concentration.
This calculator uses common conversion factors for dsDNA and ssDNA and includes the dilution factor.
Calculator
DNA A260 Concentration Calculator
Result
dsDNA concentration: 50 µg/mL
Equivalent: 50 ng/µL
Practical use
Use this calculator as a planning aid for molecular biology, biochemistry, protein science, cloning, PCR, and routine bench preparation workflows.
Results should be interpreted alongside your protocol, instrument settings, reagent quality, and laboratory safety requirements.
How to cite this tool
Citation
If this calculator supports your research, you may cite it as a web-based tool.
Abdulfattah, M. A. DNA A260 Concentration Calculator. Mustafa A Abdulfattah Academic Website. Available at: https://www.mustafaabdulfattah.com/tools/dna-a260-concentration-calculator. Accessed today.
BibTeX
@misc{abdulfattah_dna_a260_calculator_2026,
author = {Abdulfattah, Mustafa A.},
title = {DNA A260 Concentration Calculator},
year = {2026},
url = {https://www.mustafaabdulfattah.com/tools/dna-a260-concentration-calculator},
note = {Accessed today}
}FAQ
DNA A260 Concentration Calculator questions
What factor is used for dsDNA?↑
A common estimate is A260 multiplied by 50 and the dilution factor for dsDNA concentration in micrograms per mL.
What factor is used for ssDNA?↑
A common estimate is A260 multiplied by 33 and the dilution factor for ssDNA concentration in micrograms per mL.
Is ng/µL the same numeric value as µg/mL?↑
Yes. For these concentration units, 1 ng/µL equals 1 µg/mL.
Can contaminants affect A260?↑
Yes. Protein, phenol, salts, and other contaminants can influence absorbance and purity ratios.