Simple dilution planning
The dilution relationship connects stock concentration, desired concentration, final volume, and stock volume.
Use compatible concentration units so the calculator can compare stock and target concentration correctly.
Calculator
C1V1 = C2V2 Dilution Calculator
Result
Stock volume V1: 1 mL
Diluent volume: 9 mL
Final volume: 10 mL
Practical use
Use this calculator as a planning aid for molecular biology, biochemistry, protein science, cloning, PCR, and routine bench preparation workflows.
Results should be interpreted alongside your protocol, instrument settings, reagent quality, and laboratory safety requirements.
How to cite this tool
Citation
If this calculator supports your research, you may cite it as a web-based tool.
Abdulfattah, M. A. C1V1 = C2V2 Dilution Calculator. Mustafa A Abdulfattah Academic Website. Available at: https://www.mustafaabdulfattah.com/tools/dilution-calculator. Accessed today.
BibTeX
@misc{abdulfattah_dilution_calculator_2026,
author = {Abdulfattah, Mustafa A.},
title = {C1V1 = C2V2 Dilution Calculator},
year = {2026},
url = {https://www.mustafaabdulfattah.com/tools/dilution-calculator},
note = {Accessed today}
}FAQ
C1V1 = C2V2 Dilution Calculator questions
What does C1V1 = C2V2 calculate?↑
It calculates the stock volume needed to prepare a desired final concentration and volume.
What is diluent volume?↑
Diluent volume is the final volume minus the stock volume.
Can I use different concentration units?↑
Yes, if the units describe the same concentration family, such as mM and µM.
What if V1 is larger than final volume?↑
The target concentration is not reachable by dilution from the entered stock.