Agarose gel preparation
Agarose gel percentage is a weight-per-volume value.
For example, a 1 percent gel in 100 mL buffer uses 1 g agarose.
Calculator
Agarose Gel Calculator
Result
Agarose mass: 1 g
Buffer volume: 100 mL
1% gel in 100 mL = 1 g agarose
Practical use
Use this calculator as a planning aid for molecular biology, biochemistry, protein science, cloning, PCR, and routine bench preparation workflows.
Results should be interpreted alongside your protocol, instrument settings, reagent quality, and laboratory safety requirements.
How to cite this tool
Citation
If this calculator supports your research, you may cite it as a web-based tool.
Abdulfattah, M. A. Agarose Gel Calculator. Mustafa A Abdulfattah Academic Website. Available at: https://www.mustafaabdulfattah.com/tools/agarose-gel-calculator. Accessed today.
BibTeX
@misc{abdulfattah_agarose_gel_calculator_2026,
author = {Abdulfattah, Mustafa A.},
title = {Agarose Gel Calculator},
year = {2026},
url = {https://www.mustafaabdulfattah.com/tools/agarose-gel-calculator},
note = {Accessed today}
}FAQ
Agarose Gel Calculator questions
How much agarose is needed for a 1% gel?↑
A 1% gel uses 1 g agarose per 100 mL final gel volume.
What buffer volume should I use?↑
Use the final gel volume as the buffer volume before dissolving agarose.
Can I calculate 2% gels?↑
Yes. Enter the desired gel percentage and final volume.
Does this choose gel percentage for me?↑
No. Choose gel percentage based on DNA size range and your lab protocol.